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We quantified overall microorganisms working with universal bacterial primers [29] by qPCR in 114 Grownup fecal DNA samples that were analyzed for microbiota composition making use of Illumina MiSeq for 16S rRNA gene amplicon sequencing [fourteen]. The qPCR threshold cycle (Ct) values have been converted towards the estimates of bacterial genomes present in 1 g of feces being a proxy of total bacterial counts.
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This process may take various months to finish, allowing the extract to develop a deep, nuanced flavor.
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